Longevity & Aging
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Brain
Tissue-level analysis ยท GSE227359
Brain RNA-seq profile
Brain RNA-seq samples
A reproducible descriptive view of the deposited GSE227359 raw-count matrix:
matrix-total CPM, sample composition, matrix-column sums, detected genes, high-expression
ranking, and PCA. No differential-expression or causal claim is made.
Deposited matrix
GSE227359
16,816 matrix rows
9 selected samples
1 represented tissue
12,770 median genes at CPM โฅ 1
15.7M median matrix column sum
Interpretation boundary.
These are descriptive summaries of deposited raw counts normalized by the sum of all published
matrix rows in each sample. Matrix-total CPM is not a substitute for a full differential-expression
workflow, and the paper-wide 371-sample integration is not reconstructed here.
Matrix overview
What is represented in this exact slice
Counts below refer only to the selected GSE227359 columns
.
01
Species representation
Number of biological sample columns per group
02
Matrix column sums
Per-sample total across all published gene rows
Range: 6.3Mโ16.2M counts.
This is a matrix-column sum, not an independently reported sequencing depth.
Expression structure
Sample separation and dominant transcripts
Visual summaries retain replicate-level information and expose sample counts.
03
Principal component view
Exploratory structure in log2(CPM + 1)
PC1 ยท 63.9%
PC2 ยท 30.1%
PCA of centered log2(matrix-total CPM + 1), using up to 500 highest-variance genes with nonzero matrix-total CPM in at least 20% of selected samples.
04
Top mean-expression genes
Ranked by mean matrix-total CPM
ENSMUSG00000064351
18,408.4 CPM
9/9 samples at CPM โฅ 1
ENSMUSG00000064370
15,875.3 CPM
9/9 samples at CPM โฅ 1
ENSMUSG00000064367
12,882.1 CPM
8/9 samples at CPM โฅ 1
ENSMUSG00000064363
8,730.7 CPM
8/9 samples at CPM โฅ 1
ENSMUSG00000064345
8,496.0 CPM
8/9 samples at CPM โฅ 1
ENSMUSG00000064341
7,684.3 CPM
8/9 samples at CPM โฅ 1
Apoe
4,334.1 CPM
9/9 samples at CPM โฅ 1
Sparcl1
3,906.2 CPM
9/9 samples at CPM โฅ 1
Eef1a1
3,821.1 CPM
9/9 samples at CPM โฅ 1
ENSMUSG00000064354
3,415.9 CPM
4/9 samples at CPM โฅ 1
Pcsk1n
3,413.8 CPM
9/9 samples at CPM โฅ 1
Hspa8
3,253.6 CPM
9/9 samples at CPM โฅ 1
Reproducible sample audit
Every selected matrix column
The table is the text equivalent for the sample-level visualizations above.
Sample
Species group
Tissue
Replicate
Matrix column sum
Genes at CPM โฅ 1
Beaver_1_Brain
Beaver
Brain
1
15,687,136
12,770
Beaver_2_Brain
Beaver
Brain
2
15,247,495
12,666
Baboon_1_Brain
Baboon
Brain
1
15,329,036
12,823
Baboon_2_Brain
Baboon
Brain
2
16,153,874
12,823
Baboon_3_Brain
Baboon
Brain
3
15,859,251
12,702
Baboon_4_Brain
Baboon
Brain
4
14,335,439
12,728
Baboon_5_Brain
Baboon
Brain
5
15,771,755
12,792
Baboon_6_Brain
Baboon
Brain
6
16,027,795
12,825
WhiteFootedMouse_1_Brain
White Footed Mouse
Brain
1
6,326,129
11,457
Method and provenance
How this page was calculated
Normalization
Matrix-total CPM
raw count / sum of all published matrix gene rows in the sample ร 1,000,000. A gene is called detected at CPM โฅ 1.
Ranking
Descriptive mean expression
Top genes are ranked by mean CPM across the exact selected sample columns; no p-values are calculated.
Source integrity
GSE227359
SHA-256: 02ccd190468eb1709362fb90021f9cf97e48e464f7732a2d895a72c3a29d0d32