Home/ Longevity Cohort/ Published evidence
Paper evidence ยท no local matrix

New England Centenarian multi-modal atlas ('elite immunity')

eBioMedicine ยท 2023. Raw sequencing is not on this host. What follows is the open paper layer: access route, figure captions with PMC originals, and 1โ€“2 core tables transcribed from the publication.

NECS Synapse pending ยท GEO TBD PMID 37005201 PMC10114155 CC BY-NC-ND 4.0
Access truth Paper text: NECS 'will be available from Synapse'. Public reuse: NATGEN molgenis and RIKEN SC2018. Analysis scripts are on GitHub. No local NECS matrix on LACA.
Non-derivative licence. Show original figures via PMC; do not redraw UMAPs. Numeric facts below are transcribed from the paper.

Technology

Multi-modal scRNA-seq + flow validation

Material

Peripheral blood mononuclear cells (PBMC)

Local matrix

None. This page is not a donor browser.

Source note

No local NECS matrix. Use the published-evidence page for Table 1 and composition numbers. Exact participant-level age, sex and sample accession are not mapped.

Published sample accounting

Study-reported counts, not deduplicated LACA donors.

Site / layer CEN / SC Offspring Control Note
Integrated atlas 14 0 52 66 people / 102,284 cells; EL = 100โ€“119 y
New NECS 10x 7 0 2 16,082 PBMCs; Table 1

Paper figure gallery

Captions are curated locally. The images themselves stay on PMC / the publisher. This article is ND-licensed, so LACA does not rehost or recolour the panels.

Fig.1 Immune landscape at single-cell resolution UMAP of 102,284 PBMCs from 66 people, labelled by subtype and by four age groups. Fig.2 Repertoire shifts in extreme longevity Cell-type proportions, diversity statistic (F-test P=0.0001875) and age-coefficient heatmap. Fig.3 Lymphoid / myeloid resilience hierarchy Average proportions along the peripheral immune hierarchy across four age groups. Fig.4 Three gene-expression patterns Aging-related, EL-specific and aging-specific DEGs (151 age/EL genes; 387 EL vs younger).

Core published tables

Transcribed facts from the open full text. Not a re-analysis.

Table 1. New NECS samples (de-identified age range + sex)
Sample ID Age range Sex
EL1 100โ€“105 Male
EL2 105+ Female
EL3 105+ Female
EL4 100โ€“105 Female
EL5 105+ Male
EL6 105+ Male
EL7 100โ€“105 Female
YA1 44 Male
YA2 34 Female
Source: Table 1, PMC10114155.
Published composition contrasts (EL vs younger)
Metric Younger / 20โ€“89 Extreme longevity
Myeloid / lymphoid 13.8% / 86.2% 25.2% / 74.8%
Mono / DC 85.59% / 14.41% 94.58% / 5.42%
Cytotoxic / non-cytotoxic lymphocytes 30.30% / 69.70% 70.94% / 29.06%
Non-cytotoxic: CD4 T / B 90.86% / 9.41% 81.55% / 18.45%
CD4 T: memory / naive 39.46% / 60.54% 65.72% / 34.28%
cCD4TC share of PBMCs <1% 7.5% male EL; 6.0% female EL
Integrated n people / cells โ€” 66 / 102,284
DEG EL vs younger โ€” 387 (136 up / 251 down)
Source: Main text of PMC10114155.

Accessions and exits

PMC full text โ†— PubMed โ†— Publisher โ†— Synapse UH2AG064704 ยท Promised NECS deposit โ†— GitHub sc_pbmc_centenarians ยท Scripts to reproduce figures โ†— NATGEN molgenis ยท Public lifespan reuse โ†— RIKEN SC2018 ยท Japan public reuse โ†—