Rugao 'Blue Zone' Longevity Immune Remodeling

Advanced Science (Weinheim) · 2022. Raw sequencing is not on this host. What follows is the open paper layer: access route, figure captions with PMC originals, and 1–2 core tables transcribed from the publication.

Controlled access PMID 36354175 PMC9799020 CC BY 4.0

Access truth Raw sequence data are in NGDC BioProject PRJCA011218 / GSA-Human HRA002867 (controlled). No local h5ad on LACA.

Open licence. Captions and published numbers may be shown with attribution. Raw reads remain GSA-Human controlled.

Technology

scRNA-seq + TCR

Material

Peripheral blood immune cells

Local matrix

None. This page is not a donor browser.

Source note

No local single-cell matrix. Use the published-evidence page for figures and remodeling numbers. Exact participant-level age, sex and sample accession are not mapped.

Published sample accounting

Study-reported counts, not deduplicated LACA donors.

Site / layer	CEN / SC	Offspring	Control	Note
Rugao scRNA-seq	7	6	9	Ages CEN 100–108; offspring 61–83; control 60–77
Flow validation	30	30	30	Independent FACS, not the scRNA donors

Paper figure gallery

Captions are curated locally. The images themselves stay on PMC / the publisher. Open the PMC figure page for the original panel.

Fig.1 PBMC single-cell profile Study design, t-SNE of major lineages, per-participant cell counts and T-cell subclustering. Fig.2 CPC enriched, NPC depleted Log-odds enrichment plus scRNA and FACS box plots of cytotoxic vs naive-like T cells. Fig.3 Longevity vs aging T-cell maps Comparison against Zheng et al. and Mogilenko et al. aging cohorts. Fig.4 Shared CPC / NPC programmes Shared DEGs, KEGG pathways and lower aging scores in CEN and offspring T cells. Fig.5 SCENIC regulons Longevity-associated TFs including JUND, EOMES, MAFB and FOXO3. Fig.6 TCR clonal expansion Lower Shannon diversity, higher clonality and STARTRAC expansion in CEN and offspring.

Core published tables

Transcribed facts from the open full text. Not a re-analysis.

Published remodeling numbers
Metric	Value	Scope
scRNA samples	22	7 CEN + 6 offspring + 9 controls
FACS validation n	30 per group	CEN / offspring / control
CD8 share of CPC in CEN	~80%	scRNA T-cell compartment
CD4+ CTL mean in CEN	12.7% of T cells	Authors contrast with 25.3% in a supercentenarian report
Shared CPC DEGs, CEN ∩ offspring	~40%	Versus control
Enriched CD8 states in CEN/CO	GZMB+ and CMC1+	Not a further GZMK+ expansion
TCR diversity	Decreased in CEN and offspring	Shannon entropy
TCR clonality / STARTRAC	Increased in CEN and offspring	Clonal expansion

Source: PMC9799020 main text.

Accessions and exits

PMC full text ↗ PubMed ↗ Publisher ↗ PRJCA011218 · Study project ↗ HRA002867 · Raw scRNA / TCR (controlled) ↗